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Although there are some cardioprotective benefits to LC supplementation in certain situations (such as lowering all-cause mortality after MI), the formation of TMAO that goes along with it raises questions regarding long-term cardiovascular safety, particularly for people who already have heart issues [83]

A 4 l drop of sample was applied to the grid and blotted with a Vitrobot Mark IV (Thermo Fisher Scientific) operating at 4C and 100% humidity, wait time of 10 s, blot time of 4 s, and blot force of 1, before plunge-freezing into liquid ethane