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doi: 10.1038/s41392-023-01654-7
10.1016/0047-6374(84)90066-6 Mech

Immediately before measurements, protein stocks were diluted to 100 nM in buffer containing 25 mM HEPES (pH 7.4), 150 mM NaCl, and 1 mM EDTA

Subsequently, 5 mL of 0.5 M H 2 O 2 was included in the in-situ cell, and we collected the in-situ FTIR spectra at specific intervals, maintaining a reaction time of 10 min
Many suppliers source mass-produced powders with little to no quality control
