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After thoroughly mixing the extract by vortexing, the precipitated protein was removed by centrifugation (16,000 g , 5 min, 4 C) before the supernatants were analysed by HPLCMS

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10.3389/fmicb.2018.01439 77 LiS.QiC.ZhuH.YuR.XieC.PengY.et al (2019)