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Embryonic fibroblasts from Gpx4(+/) mice: A novel model for studying the role of membrane peroxidation in biological processes

Available: [9] C

Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)

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doi:10.1016/j.freeradbiomed.2008.10.040 Hassing JM, Hupka AL, Stohs SJ, Yoon PC (1979) Hepatic glutathione levels in D-penicillamine-fed ethanol-dependent rats