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The most frequently described types of infections were respiratory tract infections (RTI, n=204/715, 28.5%), among which the most common were otitis media (OM, n=119/715, 16.6%) and pneumonia (PNA, n=67/715, 9.4%), followed by bronchiolitis (n=10/715, 1.4%) and sinusitis (n=6/715, 0.8%)

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Merviel P., Cabry R., Chardon K., et al., Impact of Oocytes With CLCG on ICSI Outcomes and Their Potential Relation to Pesticide Exposure, Journal of Ovarian Research 10, no

Fifty l supernatant was mixed with 40 l 100 mM KHPO 4 (pH 7.0) and incubated for 5 min at 37 C, and then 10 l substrates (final 100 M) such as 3-mercaptopyruvate (3MP, Sigma-Aldrich), Na 2 S (Wako Pure Cheimcal Industries, Osaka, Japan) or a control H 2 O were added to incubate at 37 C for 15 min

Despite numerous reported methodologies for isolating EVs 15,16,17 , no single approach satisfies all four aspects of efficiency, scalability, accessibility and customizability simultaneously, hindering the use of EVs in further investigations, including clinical applicability