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The open field test was included to evaluate anxiety-like behavior while minimizing potential one-trial tolerance effects associated with repeated exposure to the elevated plus maze

CD8 + T cells were activated and treated with ML226 for 11 days, as previously described, incubated at 21%, 5% or 1% oxygen for 2 h, and then reactivated with anti-CD3/CD28 Dynabeads

In PD patients, nearly 80% loss of CP ferroxidase activity in the SN may lead to pro-oxidative iron accumulation, as evidenced by CP knockout mice developing parkinsonian symptoms reversible by iron chelation (56)

EVs were added to cells under the same EV and cell concentration described above

Room temperature storage: Stable for 6-12 months when sealed Keep below 25C (77F) Protect from direct sunlight Store in original packaging or wrap in foil Refrigerated storage (2-8C / 36-46F): Extends shelf life to 18-24 months Preferred for long-term storage Keep in main fridge section (not freezer, not door) Freezer storage (-20C / -4F): Maximum shelf life: 24-36 months Only for unopened sealed vials Thaw completely before reconstituting Don't repeatedly freeze and thaw Why lyophilized powder is stable: Freeze-drying removes water, which is necessary for degradation reactions