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showed that depletion in glutathione levels due to oxidative stress is related to microglial glutamate release [14]
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After being washed with phosphate buffered solution (PBS) and fixed in 4% prewarmed (37 C) paraformaldehyde for 15 min, the cells were permeabilized with 0.5% Triton X-100 for 15 min and blocked for 30 min in 10% goatserum
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